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Human CCL3L1 / LD78 ELISA Kit

SKU:
HUFI02297
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P16619
Sensitivity:
46.875pg/ml
Range:
78.125-5000pg/ml
ELISA Type:
Sandwich
Synonyms:
CCL3L1, G0S19-2, LD78 beta, SCYA3L1, chemokine, C-C motif ligand 3-like 3, 464.2, chemokine, C-C motif ligand 3-like, centromeric, G0, G1 switch regulatory protein 19-2, G0S19-2, LD78, LD78BETA, LD78.125-beta, 1-70, PAT 464.2, SCYA3L, SCYA3L1, small
Reactivity:
Human
Signaling Molecule:
Chemokine
€599
Frequently bought together:

Description

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Human CCL3L1/LD78 ELISA Kit

The Human CCL3L1 (LD78) ELISA Kit is specifically designed for the precise measurement of CCL3L1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.CCL3L1 is a key chemokine involved in immune response and inflammation, playing a critical role in various diseases such as autoimmune disorders, infectious diseases, and inflammatory conditions.

This ELISA kit enables researchers to quantify CCL3L1 levels, providing valuable insights for studying these diseases and developing potential therapeutic interventions.Overall, the Human CCL3L1 (LD78) ELISA Kit is a valuable tool for researchers looking to investigate the role of CCL3L1 in different disease processes and explore its potential as a biomarker for diagnostic and therapeutic purposes.

Product Name:Human CCL3L1 / LD78 ELISA Kit
Product Code:HUFI02297
Size:96 Assays
Alias:CCL3L1, G0S19-2, LD78 beta, SCYA3L1, chemokine, C-C motif ligand 3-like 3, 464.2, chemokine, C-C motif ligand 3-like, centromeric, G0, G1 switch regulatory protein 19-2, G0S19-2, LD78, LD78BETA, LD78.125-beta, 1-70, PAT 464.2, SCYA3L, SCYA3L1, small inducible cytokine A3-like 1, Small-inducible cytokine A3-like 1, Tonsillar lymphocyte LD78 beta protein
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human CCL3L1 concentrations in serum plasma and other biological fluids.
Sensitivity:46.875pg/ml
Range:78.125-5000pg/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human CCL3L1 and the recovery rates were calculated by comparing the measured value to the expected amount of Human CCL3L1 in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)87-10596
EDTA plasma(n=5)86-10594
UFH plasma(n=5)89-9995
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human CCL3L1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)85-101%86-104%87-99%
EDTA plasma(n=5)94-99%87-97%83-100%
UFH plasma(n=5)82-96%82-89%81-99%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotP16619
UniProt Protein Function:CCL3L1: Chemotactic for lymphocytes and monocytes. Is a ligand for CCR1, CCR3 and CCR5. Is an inhibitor of HIV-1-infection. The processed form LD78-beta(3-70) shows a 20-fold to 30-fold higher chemotactic activity and is a very potent inhibitor of HIV-1- infection. LD78-beta(3-70) is also a ligand for CCR1, CCR3 and CCR5. Belongs to the intercrine beta (chemokine CC) family.
UniProt Protein Details:Protein type:Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis; Chemokine

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: extracellular space; extracellular region

Molecular Function:chemokine activity

Biological Process: negative regulation of cell proliferation; immune response; inflammatory response

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To

NCBI Summary:This gene is one of several cytokine genes that are clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins that function in inflammatory and immunoregulatory processes. The protein encoded by this gene binds to several chemokine receptors, including chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5). CCR5 is a co-receptor for HIV, and binding of this protein to CCR5 inhibits HIV entry. The copy number of this gene varies among individuals, where most individuals have one to six copies, and a minority of individuals have zero or more than six copies. There are conflicting reports about copy number variation of this gene and its correlation to disease susceptibility.[provided by RefSeq, Apr 2014]
UniProt Code:P16619
NCBI GenInfo Identifier:127077
NCBI Gene ID:414062
NCBI Accession:P16619.1
UniProt Secondary Accession:P16619,Q3B7A8, Q53YA5, Q96I68, A6NGK6, B2RUX4,
UniProt Related Accession:P16619
Molecular Weight:93
NCBI Full Name:C-C motif chemokine 3-like 1
NCBI Synonym Full Names:chemokine (C-C motif) ligand 3-like 3
NCBI Official Symbol:CCL3L3  
NCBI Official Synonym Symbols:LD78; 464.2; SCYA3L; G0S19-2; SCYA3L1; D17S1718; LD78BETA  
NCBI Protein Information:C-C motif chemokine 3-like 1; LD78-beta(1-70); small inducible cytokine A3-like 1; G0/G1 switch regulatory protein 19-2; tonsillar lymphocyte LD78 beta protein; chemokine (C-C motif) ligand 3-like, centromeric
UniProt Protein Name:C-C motif chemokine 3-like 1
UniProt Synonym Protein Names:G0/G1 switch regulatory protein 19-2; LD78-beta(1-70); PAT 464.2; Small-inducible cytokine A3-like 1; Tonsillar lymphocyte LD78 beta proteinCleaved into the following 2 chains:LD78-beta(3-70); LD78-beta(5-70)
Protein Family:C-C motif chemokine
UniProt Gene Name:CCL3L1  
UniProt Entry Name:CL3L1_HUMAN

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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