Human CBX5 Recombinant Protein (RPPB5870)
- SKU:
- RPPB5870
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P45973
Description
| Product Name: | Human CBX5 Recombinant Protein |
| Product Code: | RPPB5870 |
| Size: | 20µg |
| Species: | Human |
| Target: | CBX5 |
| Synonyms: | Chromobox homolog 5 (HP1 alpha homolog, Drosophila), Heterochromatin protein 1 homolog alpha, HP1Hs alpha, HP1 alpha homolog. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The CBX5 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM Nacl, 1mM DTT and 30% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMGKKTK RTADSSSSED EEEYVVEKVL DRRVVKGQVE YLLKWKGFSE EHNTWEPEKN LDCPELISEF MKKYKKMKEG ENNKPREKSE SNKRKSNFSN SADDIKSKKK REQSNDIARG FERGLEPEKI IGATDSCGDL MFLMKWKDTD EADLVLAKEA NVKCPQIVIA FYEERLTWHA YPEDAENKEK ETAKS |
CBX5 belongs to the heterochromatin protein family. CBX5 is enriched in the heterochromatin and linked with centromeres. CBX5 takes part in the creation of functional kinetochore by interaction with important kinetochore proteins. CBX5 has a pseudogene situated on chromosome 3. Additionaly, The CBX5 proteins reconnect with chromatin at the end of mitosis, as Histone H3 is dephosphorylated.
CBX5 Human Recombinant produced in E. coli is a single polypeptide chain containing 215 amino acids (1-191) and having a molecular mass of 24.8kDa.CBX5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | HP1 alpha: Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Interacts with SUV420H1 and SUV420H2. Interacts with HP1BP3. Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys- 9'. Interacts with BAHD1, MIS12 and DSN1. Interacts with POGZ; POGZ and PXVXL motif-containing proteins such as INCENP and TRIM28 compete for interaction with CBX5. Interacts with INCENP and TRIM24. Interacts with JC virus agnoprotein; this interaction induces the dissociation of CBX5 from LBR, resulting in destabilization of the nuclear envelope. Interacts with CHAMP1. Interacts with ASXL1. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 12q13.13 Cellular Component: kinetochore; nucleoplasm; PML body; histone methyltransferase complex; transcriptional repressor complex; histone deacetylase complex; nucleolus; nuclear envelope; nuclear heterochromatin; nucleus; chromocenter Molecular Function:protein binding, bridging; protein binding; protein homodimerization activity; histone deacetylase binding; chromatin binding; methylated histone residue binding Biological Process: viral reproduction; blood coagulation; negative regulation of transcription, DNA-dependent |
| NCBI Summary: | This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P45973 |
| NCBI GenInfo Identifier: | 1170338 |
| NCBI Gene ID: | 23468 |
| NCBI Accession: | P45973.1 |
| UniProt Secondary Accession: | P45973,B2R8T9, |
| UniProt Related Accession: | P45973 |
| Molecular Weight: | 191 |
| NCBI Full Name: | Chromobox protein homolog 5 |
| NCBI Synonym Full Names: | chromobox homolog 5 |
| NCBI Official Symbol: | CBX5 |
| NCBI Official Synonym Symbols: | HP1; HP1A; HEL25 |
| NCBI Protein Information: | chromobox protein homolog 5; HP1-ALPHA; HP1Hs alpha; antigen p25; HP1 alpha homolog; epididymis luminal protein 25; heterochromatin protein 1-alpha; heterochromatin protein 1 homolog alpha; chromobox homolog 5 (HP1 alpha homolog, Drosophila) |
| UniProt Protein Name: | Chromobox protein homolog 5 |
| UniProt Synonym Protein Names: | Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha |
| Protein Family: | Chromobox protein |
| UniProt Gene Name: | CBX5 |
| UniProt Entry Name: | CBX5_HUMAN |
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