Human CBG (Corticosteroid Binding Globulin) ELISA Kit
The Human CBG (Corticosteroid Binding Globulin) ELISA Kit is specifically designed for the precise measurement of CBG levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures consistent and accurate results, making it ideal for various research applications.CBG is a key protein responsible for binding and transporting cortisol, a hormone crucial for regulating metabolism, immune response, and stress. Imbalances in CBG levels have been associated with various medical conditions, including endocrine disorders, inflammation, and autoimmune diseases.
Therefore, studying CBG levels can provide valuable insights into these conditions and help in the development of potential therapeutic interventions.This ELISA kit provides researchers with a reliable tool to investigate the role of CBG in health and disease, ultimately contributing to advancements in understanding and treating related disorders.
Product Name:
Human CBG (Corticosteroid Binding Globulin) ELISA Kit
SKU:
HUES03328
Target:
Human CBG (Corticosteroid Binding Globulin) ELISA Kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CBG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CBG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CBG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CBG. You can calculate the concentration of Human CBG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
88-102
98-114
Average (%)
98
95
105
1:4
Range (%)
89-101
85-98
85-100
Average (%)
95
91
92
1:8
Range (%)
87-100
85-96
85-99
Average (%)
94
91
91
1:16
Range (%)
88-100
84-98
88-100
Average (%)
94
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-102
93
EDTA plasma (n=5)
89-101
95
Cell culture media (n=5)
88-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
21.53
40.48
142.3
22.23
39.21
156.49
Standard deviation
1.32
1.91
6.4
1.3
1.91
6.78
C V (%)
6.13
4.72
4.5
5.85
4.87
4.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CBG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CBG in samples. No significant cross-reactivity or interference between Human CBG and analogues was observed.
