Human Cationic Amino Acid Transporter 2 (CAT2) ELISA Kit
The Human Cationic Amino Acid Transporter 2 (CAT2) ELISA Kit is a powerful tool for accurately measuring CAT2 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and consistent results, making it ideal for various research applications.CAT2 is a key protein responsible for transporting cationic amino acids across cell membranes, playing a crucial role in cellular metabolism and function. Dysregulation of CAT2 activity has been implicated in a variety of diseases, including immune disorders, neurological conditions, and metabolic syndromes.
The CAT2 ELISA Kit allows researchers to study the role of CAT2 in these diseases and potentially develop targeted therapies to mitigate its effects.Overall, the Human CAT2 ELISA Kit is a valuable tool for researchers seeking to investigate the functions and implications of CAT2 in human biology and disease. Its precision and reliability make it an essential component of any laboratory studying cationic amino acid transport mechanisms.
Product Name:
Human Cationic Amino Acid Transporter 2 (CAT2) ELISA Kit
Product Code:
HUDL00441
Size:
96 Assays
Target:
CAT2
Synonyms:
SLC7A2, ATRC2, CAT-2, HCAT2, Low Affinity Cationic Amino Acid Transporter 2, Solute Carrier Family 7 Member 2,Cationic Amino Acid Transporter,y+ System
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
