The Human Caspase-3 ELISA Kit is specifically designed for the precise quantification of Caspase-3 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Caspase-3 is a key enzyme involved in apoptosis, playing a crucial role in programmed cell death. Dysregulation of Caspase-3 has been implicated in various diseases such as cancer, neurodegenerative disorders, and autoimmune conditions, making it an important biomarker for studying these diseases and potential therapeutic interventions.
With its reliable performance and ease of use, the Human Caspase-3 ELISA Kit is an essential tool for researchers looking to understand the role of Caspase-3 in disease pathogenesis and develop targeted treatments.
Product Name:
Human CASP3 (Caspase 3) ELISA Kit
SKU:
HUES01304
Target:
Human CASP3 (Caspase 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CASP3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CASP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CASP3. You can calculate the concentration of Human CASP3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
90-103
98-113
Average (%)
92
97
105
1:4
Range (%)
86-98
87-98
82-95
Average (%)
93
92
88
1:8
Range (%)
93-105
85-95
82-92
Average (%)
99
90
87
1:16
Range (%)
87-99
82-96
84-94
Average (%)
93
88
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
88-103
94
Cell culture media (n=5)
93-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.97
2.79
7.18
0.94
2.57
7.67
Standard deviation
0.05
0.12
0.32
0.06
0.14
0.27
C V (%)
5.15
4.3
4.46
6.38
5.45
3.52
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CASP3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CASP3 in samples. No significant cross-reactivity or interference between Human CASP3 and analogues was observed.
