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Human Casein Alpha (CSN1) ELISA Kit

SKU:
HUDL00698
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P47710
Sensitivity:
0.55ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
CASa,CSN1,CSN1S1,Casein Alpha S1,Caseinate,Caseine/cas?©ine/Kasein/case??na,Alpha-S1-casein,Casoxin-D
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants, Breast Milk And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Casein Alpha (CSN1) ELISA Kit

The Human Casein Alpha (CSN1) ELISA Kit is specifically designed for the quantitative measurement of human casein alpha levels in serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, allowing for accurate and reproducible results in various research applications.Casein alpha is a key component of milk protein and plays a vital role in providing essential nutrients to infants and maintaining overall health.

Abnormal levels of casein alpha have been linked to various health conditions such as lactation disorders, digestive issues, and autoimmune diseases, making it a valuable biomarker for studying and understanding these conditions.With easy-to-follow protocols and reliable performance, the Human Casein Alpha (CSN1) ELISA Kit is a valuable tool for researchers and clinicians alike looking to explore the role of casein alpha in health and disease.

Product Name:Human Casein Alpha (CSN1) ELISA Kit
Product Code:HUDL00698
Size:96 Assays
Target:CSN1
Synonyms:CASa, CSN1, CSN1S1, Casein Alpha S1, Caseinate, Caseine/caséine/Kasein/caseína, Alpha-S1-casein, Casoxin-D
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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