Human Cartilage Intermediate Layer Protein 2 (CILP2) ELISA Kit
The Human CILP2 (Cartilage Intermediate Layer Protein 2) ELISA Kit is specifically designed for the precise measurement of CILP2 levels in human samples including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable tool for researchers in various fields.CILP2 is a vital protein found in the intermediate layer of cartilage, playing a crucial role in maintaining cartilage integrity and function.
Its dysregulation has been linked to various musculoskeletal disorders such as osteoarthritis and rheumatoid arthritis, highlighting its significance as a biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human CILP2 ELISA Kit offers a reliable and efficient method for quantifying CILP2 levels, enabling researchers to gain valuable insights into the pathophysiology of cartilage-related diseases and potentially uncover novel treatment strategies.
Product Name:
Human Cartilage Intermediate Layer Protein 2 (CILP2) ELISA Kit
Product Code:
HUDL00574
Size:
96 Assays
Target:
CILP2
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
