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Human Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6) ELISA Kit

SKU:
HUDL00525
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P40199
Sensitivity:
9.68pg/mL
Range:
23.437-1500pg/mL
ELISA Type:
Sandwich
Synonyms:
CD66c,NCA,CD66-C,CEAL,Non-Specific Cross Reacting Antigen,Normal cross-reacting antigen
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
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Description

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Human Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6) ELISA Kit

The Human CEACAM6 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6) ELISA Kit is a reliable tool for detecting CEACAM6 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.CEACAM6 is a cell adhesion molecule associated with cancer progression and metastasis, making it a valuable biomarker for studying cancer development and potential therapies.

Its expression is also linked to inflammatory diseases and autoimmune disorders, highlighting its importance in understanding immune responses and disease mechanisms.With its advanced technology and easy-to-follow protocol, the Human CEACAM6 ELISA Kit is an essential tool for researchers investigating the role of CEACAM6 in health and disease.

Product Name:Human Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6) ELISA Kit
Product Code:HUDL00525
Size:96 Assays
Target:CEACAM6
Synonyms:CD66c, NCA, CD66-C, CEAL, Non-Specific Cross Reacting Antigen, Normal cross-reacting antigen
Detection Method:Sandwich
Range:23.437-1,500pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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