null

Human Carboxylesterase 1 (CES1) ELISA Kit

SKU:
HUDL00537
Product Type:
ELISA Kit
Sensitivity:
0.072ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ACAT,CEH,CES2,HMSE1,PCE1,SES1,TGH,Retinyl ester hydrolase,Serine esterase 1,Triacylglycerol hydrolase,Egasyn,Cocaine carboxylesterase,Liver Carboxylesterase 1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Carboxylesterase 1 (CES1) ELISA Kit

The Human Carboxylesterase-1 (CES1) ELISA Kit is specifically designed for the precise and reliable detection of CES1 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an ideal tool for a variety of research applications.CES1 is an important enzyme involved in the metabolism of various drugs and xenobiotics, as well as in lipid metabolism.

Dysregulation of CES1 activity has been linked to drug resistance, toxicity, and metabolic disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic strategies.Overall, the Human CES1 ELISA Kit offers researchers a robust and efficient method for quantifying CES1 levels in human samples, providing valuable insights into its role in drug metabolism and physiological processes.

Product Name:Human Carboxylesterase 1 (CES1) ELISA Kit
Product Code:HUDL00537
Size:96 Assays
Target:CES1
Synonyms:ACAT, CEH, CES2, HMSE1, PCE1, SES1, TGH, Retinyl ester hydrolase, Serine esterase 1, Triacylglycerol hydrolase, Egasyn, Cocaine carboxylesterase, Liver Carboxylesterase 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products