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Human Carbonic Anhydrase VI (CA6) ELISA Kit

SKU:
HUDL00402
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P23280
Sensitivity:
29.3pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
CA-VI,CAVI,GUSTIN,Carbonate dehydratase VI,Salivary carbonic anhydrase,Secreted carbonic anhydrase
Reactivity:
Human
Sample Type:
Serum, Plasma, Saliva And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Carbonic Anhydrase VI (CA6) ELISA Kit

The Human Carbonic Anhydrase VI (CA6) ELISA Kit is specifically designed for the accurate measurement of CA6 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, providing researchers with reliable and reproducible results for a variety of research applications.Carbonic anhydrase VI is an important enzyme involved in maintaining acid-base balance in the body and is found in saliva and other secretory fluids.

Its dysregulation has been linked to various diseases such as Sjögren's syndrome and oral cavity disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions. With the Human CA6 ELISA Kit, researchers can confidently measure CA6 levels in human samples, gaining valuable insights into its role in disease pathogenesis and potential diagnostic and therapeutic applications.

Product Name:Human Carbonic Anhydrase VI (CA6) ELISA Kit
Product Code:HUDL00402
Size:96 Assays
Target:CA6
Synonyms:CA-VI, CAVI, GUSTIN, Carbonate dehydratase VI, Salivary carbonic anhydrase, Secreted carbonic anhydrase
Detection Method:Sandwich
Range:62.5-4,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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