Human Carbonic Anhydrase III, Muscle Specific (CA3) ELISA Kit
The Human Carbonic Anhydrase III (CA3) Muscle Specific ELISA Kit is a powerful tool for accurately measuring levels of CA3 in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides dependable and consistent results, making it well-suited for a variety of research applications.Carbonic anhydrase III is an important enzyme found predominantly in skeletal muscle and plays a key role in various physiological processes, such as pH regulation and lipid metabolism.
Abnormal levels of CA3 have been linked to conditions like metabolic disorders, muscular dystrophy, and liver disease, highlighting its significance as a potential biomarker for disease diagnosis and monitoring.By utilizing the Human Carbonic Anhydrase III Muscle Specific ELISA Kit, researchers can gain valuable insights into the role of CA3 in health and disease, ultimately paving the way for the development of innovative therapeutic strategies.
Product Name:
Human Carbonic Anhydrase III, Muscle Specific (CA3) ELISA Kit
Product Code:
HUDL00398
Size:
96 Assays
Target:
CA3
Synonyms:
CA-III, CAIII, Car3, Carbonate dehydratase III
Detection Method:
Sandwich
Range:
3.125-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
