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Human Cannabinoid R1 / CB1 / CNR1 ELISA Kit

SKU:
HUFI02336
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P21554
Sensitivity:
0.094ng/ml
Range:
0.156-10ng/ml
ELISA Type:
Sandwich
Synonyms:
CNR1, CANN6, CB1, CB1A, CB1K5, CB1R, CB-R, CNR1, SKR6R, CANN6CB1R, cannabinoid receptor 1, brain, CB1A, CB1K5, CB-RCB1cannabinoid receptor 1, central cannabinoid receptor, CNR
Reactivity:
Human
Research Area:
Cell Biology
€599
Frequently bought together:

Description

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Human Cannabinoid R1/CB1/CNR1 ELISA Kit

The Human Cannabinoid Receptor 1 (CB1) ELISA Kit is a powerful tool for accurately measuring levels of CB1 protein in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.CB1, also known as the Cannabinoid Receptor 1, is a key protein involved in the endocannabinoid system, playing a crucial role in various physiological functions such as appetite regulation, pain perception, and memory.

Dysregulation of the CB1 receptor has been implicated in conditions like obesity, neuropathic pain, and addiction, making it a valuable target for drug development and therapeutic interventions.By utilizing the Human CB1 ELISA Kit, researchers can gain valuable insights into the role of CB1 in health and disease, paving the way for advancements in medical research and the development of novel treatment strategies.

Product Name:Human Cannabinoid R1 / CB1 / CNR1 ELISA Kit
Product Code:HUFI02336
Size:96 Assays
Alias:CNR1, CANN6, CB1, CB1A, CB1K5, CB1R, CB-R, CNR1, SKR6R, CANN6CB1R, cannabinoid receptor 1, brain, CB1A, CB1K5, CB-RCB1cannabinoid receptor 1, central cannabinoid receptor, CNR
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human CNR1 concentrations in serum plasma and other biological fluids.
Sensitivity:0.094ng/ml
Range:0.156-10ng/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human CNR1 and the recovery rates were calculated by comparing the measured value to the expected amount of Human CNR1 in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)87-10395
EDTA plasma(n=5)87-10494
UFH plasma(n=5)92-10097
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human CNR1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)85-103%86-94%87-104%
EDTA plasma(n=5)87-100%88-99%84-87%
UFH plasma(n=5)83-98%80-99%85-98%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotP21554
UniProt Protein Function:CNR1: Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:Protein type:GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 6q14-q15

Cellular Component: growth cone; intracellular membrane-bound organelle; integral to plasma membrane; axon; plasma membrane

Molecular Function:cannabinoid receptor activity; drug binding

Biological Process: response to nicotine; negative regulation of mast cell activation; maternal process involved in pregnancy; negative regulation of fatty acid beta-oxidation; regulation of synaptic transmission, glutamatergic; negative regulation of nitric-oxide synthase activity; positive regulation of apoptosis; axonal fasciculation; response to morphine; positive regulation of acute inflammatory response to antigenic stimulus; response to lipopolysaccharide; negative regulation of action potential; sensory perception of pain; glucose homeostasis; response to cocaine; regulation of synaptic transmission, GABAergic; memory; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein signaling, coupled to cyclic nucleotide second messenger; response to ethanol; positive regulation of fever; spermatogenesis; negative regulation of blood pressure; negative regulation of dopamine secretion; regulation of insulin secretion; response to nutrient; positive regulation of blood pressure; aging

NCBI Summary:This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, May 2009]
UniProt Code:P21554
NCBI GenInfo Identifier:115562
NCBI Gene ID:1268
NCBI Accession:P21554.1
UniProt Secondary Accession:P21554,Q13949, Q495Z0, Q4PLI4, Q4VBM6, Q5JVL5, Q5UB37 Q9UNN0, B2R9T4, E1P512,
UniProt Related Accession:P21554
Molecular Weight:Observed MW: 53kDaCalculated MW: 45kDa/49kDa/52kDa
NCBI Full Name:Cannabinoid receptor 1
NCBI Synonym Full Names:cannabinoid receptor 1 (brain)
NCBI Official Symbol:CNR1
NCBI Official Synonym Symbols:CB1; CNR; CB-R; CB1A; CB1R; CANN6; CB1K5
NCBI Protein Information:cannabinoid receptor 1; central cannabinoid receptor
UniProt Protein Name:Cannabinoid receptor 1
UniProt Synonym Protein Names:CANN6
Protein Family:Cannabinoid receptor
UniProt Gene Name:CNR1
UniProt Entry Name:CNR1_HUMAN

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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