null

Human CAMK2A / CaMKII alpha protein CaM Kinase II alpha ELISA Kit

SKU:
HUFI02287
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9UQM7
Sensitivity:
0.094ng/ml
Range:
0.156-10ng/ml
ELISA Type:
Sandwich
Synonyms:
CAMK2alpha, CAMKA
Reactivity:
Human
Research Area:
Cell Biology
$719
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

Human CAMK2A/CaMKII alpha protein CaM Kinase II alpha ELISA Kit

The Human CAMK2A (CAMKII Alpha) ELISA Kit is specifically designed for the accurate measurement of CAMKII alpha protein levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing precise and reproducible results, making it an invaluable tool for a variety of research purposes.CAMKII alpha is a vital protein involved in various cellular processes, including cell signaling, synaptic plasticity, and gene expression regulation.

Dysregulation of CAMKII alpha has been implicated in neurological disorders, cardiac diseases, and cancer, highlighting its significance as a biomarker for studying these conditions and exploring potential therapeutic interventions.For researchers and scientists interested in investigating CAMKII alpha's role in health and disease, the Human CAMK2A ELISA Kit provides a reliable and efficient method for quantifying protein levels and advancing our understanding of its functions.

Product Name:Human CAMK2A / CaMKII alpha protein CaM Kinase II alpha ELISA Kit
Product Code:HUFI02287
Size:96 Assays
Alias:CAMK2alpha, CAMKA
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human CAMK2alpha concentrations in serum plasma and other biological fluids.
Sensitivity:0.094ng/ml
Range:0.156-10ng/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human CAMK2alpha and the recovery rates were calculated by comparing the measured value to the expected amount of Human CAMK2alpha in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)85-10093
EDTA plasma(n=5)86-9893
UFH plasma(n=5)87-10194
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human CAMK2alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)85-100%88-105%87-99%
EDTA plasma(n=5)83-97%86-95%82-97%
UFH plasma(n=5)80-98%87-94%87-100%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotQ9UQM7
UniProt Protein Function:CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:Protein type:Protein kinase, CAMK; EC 2.7.11.17; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CAMK group; CAMK2 family

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: cytosol; mitochondrion; nucleoplasm; nucleus; plasma membrane

Molecular Function:calmodulin binding; glutamate receptor binding; kinase activity; protein binding; protein homodimerization activity; protein serine/threonine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: activation of NF-kappaB transcription factor; calcium ion transport; G1/S transition of mitotic cell cycle; MAPKKK cascade; peptidyl-serine phosphorylation; positive regulation of calcium ion transport; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of neuronal synaptic plasticity; regulation of neurotransmitter secretion; Wnt receptor signaling pathway, calcium modulating pathway

NCBI Summary:The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]
UniProt Code:Q9UQM7
NCBI GenInfo Identifier:296434552
NCBI Gene ID:815
NCBI Accession:Q9UQM7.2
UniProt Secondary Accession:Q9UQM7,Q9UL21, Q9Y2H4, Q9Y352,
UniProt Related Accession:Q9UQM7
Molecular Weight:55,320 Da
NCBI Full Name:Calcium/calmodulin-dependent protein kinase type II subunit alpha
NCBI Synonym Full Names:calcium/calmodulin dependent protein kinase II alpha
NCBI Official Symbol:CAMK2A
NCBI Official Synonym Symbols:CAMKA
NCBI Protein Information:calcium/calmodulin-dependent protein kinase type II subunit alpha
UniProt Protein Name:Calcium/calmodulin-dependent protein kinase type II subunit alpha
Protein Family:Calcium/calmodulin-dependent protein kinase
UniProt Gene Name:CAMK2A
UniProt Entry Name:KCC2A_HUMAN

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

Related Products