The Human Calnexin (CNX) ELISA Kit from AssayGenie is specifically designed to accurately detect levels of calnexin in human samples, including serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reliable and reproducible results, making it an excellent tool for a wide range of research applications.Calnexin is a critical protein involved in protein folding and quality control in the endoplasmic reticulum. Dysregulation of calnexin has been linked to various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.
Thus, measuring calnexin levels can provide valuable insights into these diseases and aid in the development of potential therapeutic strategies.By utilizing the Human Calnexin (CNX) ELISA Kit, researchers can gain a better understanding of calnexin's role in disease pathogenesis and potentially identify novel targets for intervention. With its ease of use and reliable performance, this kit is an invaluable tool for studying calnexin biology and its implications in human health.
Product Name:
Human Calnexin (CNX) ELISA Kit
Product Code:
HUDL00613
Size:
96 Assays
Target:
CNX
Synonyms:
CANX, CNX, IP90, P90, Major Histocompatibility Complex Class I Antigen-Binding Protein p88
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
