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Human CAIII Recombinant Protein (RPPB1461)

SKU:
RPPB1461
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P07451
Research Area:
Enzymes
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Description

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Product Name:Human CAIII Recombinant Protein
Product Code:RPPB1461
Size:10µg
Species:Human
Target:CAIII
Synonyms:Car3, CAIII, Carbonic anhydrase 3, EC 4.2.1.1, Carbonic anhydrase III, Carbonate dehydratase III, CA-III.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered blue solution.
Formulation:Supplied in 10mM Tris-HCl (pH 8), 250mM NaCl, 0.5mM DTT, 1.5mM Cysteine, and 50% Glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by�SDS-PAGE.

Carbonic anhydrase (carbonate dehydratase) is a family of metalloenzymes (enzymes that contain one or more metal atoms as a functional component of the enzyme) that catalyze the rapid (and reversible) conversion of carbon dioxide to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. Carbonic anhydrase greatly increases the rate of the reaction, with typical catalytic rates of the different forms of this enzyme ranging between 104 and 106 reactions per second. The active site of most carbonic anhydrases contains a zincion. CAIII is a cytoplasmic isoenzyme, but is released into the circulation following injury.

Carbonic anhydrase III Human Recombinant produced in E.Coli, and having a molecular mass of 33.9 kDa. CAIII is expressed with an amino-terminal hexahistidine tag.The CA-III is purified by proprietary chromatographic techniques.

UniProt Protein Function:CA3: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.
UniProt Protein Details:

Protein type:Energy Metabolism - nitrogen; EC 4.2.1.1; Lyase

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: cytosol

Molecular Function:carbonate dehydratase activity; zinc ion binding; nickel ion binding

Biological Process: response to ethanol; bicarbonate transport; one-carbon compound metabolic process; response to oxidative stress

NCBI Summary:Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008]
UniProt Code:P07451
NCBI GenInfo Identifier:134047703
NCBI Gene ID:761
NCBI Accession:P07451.3
UniProt Secondary Accession:P07451,O60842, B2R867, B3KUC8,
UniProt Related Accession:P07451
Molecular Weight:260
NCBI Full Name:Carbonic anhydrase 3
NCBI Synonym Full Names:carbonic anhydrase III, muscle specific
NCBI Official Symbol:CA3��
NCBI Official Synonym Symbols:Car3; CAIII��
NCBI Protein Information:carbonic anhydrase 3; CA-III; HEL-S-167mP; carbonate dehydratase III; epididymis secretory sperm binding protein Li 167mP
UniProt Protein Name:Carbonic anhydrase 3
UniProt Synonym Protein Names:Carbonate dehydratase III; Carbonic anhydrase III; CA-III
Protein Family:Carbonic anhydrase
UniProt Gene Name:CA3��
UniProt Entry Name:CAH3_HUMAN

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