Human cADPRH/CD38 (Cyclic ADP Ribose Hydrolase) ELISA Kit
The Human CADPRH (CD38 Cyclic ADP-Ribose Hydrolase) ELISA Kit is a highly sensitive and specific assay designed for the detection of CADPRH levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it a valuable tool for researchers in various fields.CADPRH, also known as CD38 Cyclic ADP-Ribose Hydrolase, is an enzyme involved in the metabolism of cyclic ADP-ribose, a molecule that plays a role in calcium signaling and immune response regulation. Dysregulation of CADPRH has been implicated in various diseases, including cancer, inflammatory disorders, and neurodegenerative diseases.
The Human CADPRH ELISA Kit allows for the quantification of CADPRH levels, enabling researchers to study its role in disease pathogenesis and potentially develop novel therapeutic strategies.Overall, the Human CADPRH ELISA Kit is an essential tool for researchers interested in studying the function of CADPRH and its implications in health and disease. Its high sensitivity and specificity make it a reliable choice for a wide range of research applications.
Product Name:
Human cADPRH/CD38 (Cyclic ADP Ribose Hydrolase) ELISA Kit
SKU:
HUES02628
Target:
Human cADPRH/CD38 (Cyclic ADP Ribose Hydrolase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.56 ng/mL
Detection range:
0.94-60 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human cADPRH/CD38. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human cADPRH/CD38 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human cADPRH/CD38, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human cADPRH/CD38. You can calculate the concentration of Human cADPRH/CD38 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
90-103
92-106
Average (%)
92
96
98
1:4
Range (%)
93-108
85-101
83-97
Average (%)
99
92
90
1:8
Range (%)
88-100
79-90
90-101
Average (%)
95
85
95
1:16
Range (%)
89-102
81-94
83-97
Average (%)
96
87
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
93
EDTA plasma (n=5)
95-111
101
Cell culture media (n=5)
95-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.15
6.75
27.89
3.33
6.48
29.83
Standard deviation
0.16
0.39
1.52
0.2
0.33
1.34
C V (%)
5.08
5.78
5.45
6.01
5.09
4.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human cADPRH/CD38 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human cADPRH/CD38 in samples. No significant cross-reactivity or interference between Human cADPRH/CD38 and analogues was observed.
