null

Human Cadherin 26 (CDH26) ELISA Kit

SKU:
HUDL00506
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8IXH8
Sensitivity:
0.065ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
VR20,Cadherin-like protein 26
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Human Cadherin 26 (CDH26) ELISA Kit

The Human Cadherin-26 (CDH26) ELISA Kit is a powerful tool for the quantitative measurement of CDH26 levels in human biological samples. This kit is designed with precision and accuracy in mind, providing researchers with reliable data for a variety of applications.CDH26 is a cell adhesion molecule that plays a critical role in various cellular processes, including cell-cell adhesion and signaling. Dysregulation of CDH26 has been linked to several diseases, making it an important biomarker for research and clinical studies.

With its high sensitivity and specificity, the Human Cadherin-26 ELISA Kit offers a dependable method for exploring the role of CDH26 in health and disease. Whether investigating cancer, developmental disorders, or other conditions, this kit is an essential tool for advancing scientific knowledge and developing potential therapeutic strategies.

Product Name:Human Cadherin 26 (CDH26) ELISA Kit
Product Code:HUDL00506
Size:96 Assays
Target:CDH26
Synonyms:VR20, Cadherin-like protein 26
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products