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Human Cadherin 16 (CDH16) ELISA Kit

SKU:
HUDL00504
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O75309
Sensitivity:
0.038ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
Cadherin,KSP,Kidney-specific cadherin
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Cadherin 16 (CDH16) ELISA Kit

The Human Cadherin-16 (CDH16) ELISA Kit is specifically designed for the precise measurement of CDH16 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit offers dependable and reproducible results, making it an excellent choice for various research purposes.CDH16, also known as Ksp-cadherin, is a crucial cell adhesion molecule that plays a key role in cell-cell adhesion and signaling processes.

Its dysregulation has been linked to various diseases, including cancer and kidney disorders. Therefore, measuring CDH16 levels can provide valuable insights into disease progression and potential therapeutic targets.Overall, the Human Cadherin-16 (CDH16) ELISA Kit is an essential tool for researchers studying cell adhesion mechanisms, disease development, and potential treatment options.

Product Name:Human Cadherin 16 (CDH16) ELISA Kit
Product Code:HUDL00504
Size:96 Assays
Target:CDH16
Synonyms:Cadherin,KSP, Kidney-specific cadherin
Detection Method:Sandwich
Range:0.156-10 ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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