Human CA3 (Carbonic Anhydrase III, Muscle Specific) CLIA Kit
The Human CA3 (Carbonic Anhydrase III) Muscle-Specific CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of CA3 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.Carbonic Anhydrase III is a muscle-specific enzyme that plays a critical role in acid-base balance and ion transport in skeletal muscle.
Its levels can be altered in various disease conditions such as muscular disorders, metabolic diseases, and cancer, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human CA3 Muscle-Specific CLIA Kit offers researchers a powerful tool for investigating the role of CA3 in human physiology and pathology, with the potential for advancing our understanding of muscle function and disease mechanisms.
Product Name:
Human CA3 (Carbonic Anhydrase III, Muscle Specific) CLIA Kit
SKU:
HUES00259
Target:
Human CA3 (Carbonic Anhydrase III, Muscle Specific)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CA3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CA3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CA3. You can calculate the concentration of Human CA3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
92-106
90-102
Average (%)
102
98
97
1:4
Range (%)
83-99
88-103
91-103
Average (%)
90
94
96
1:8
Range (%)
100-114
101-117
92-107
Average (%)
105
107
100
1:16
Range (%)
99-115
99-113
85-99
Average (%)
106
107
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
105
EDTA plasma (n=5)
99-115
106
Cell culture media (n=5)
101-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.95
3.5
17.49
1.85
3.63
18.82
Standard deviation
0.17
0.26
1.81
0.16
0.29
1.5
C V (%)
8.72
7.43
10.35
8.65
7.99
7.97
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CA3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CA3 in samples. No significant cross-reactivity or interference between Human CA3 and analogues was observed.
