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Human CA1 Recombinant Protein (RPPB1456)

SKU:
RPPB1456
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P00915
Research Area:
Enzymes
$371
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Description

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Product Name:Human CA1 Recombinant Protein
Product Code:RPPB1456
Size:10µg
Species:Human
Target:CA1
Synonyms:CA1, CA-I, CAB.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:CA1 Human protein (1mg/ml) is formulated in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F
Biological Activity:Specific activity is > 300pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.

CA, also known as carbonic anhydrase is an enzyme. Its main function revolves around the CO2 + H2O HCO3- + H+ (conversion of carbon dioxide to bicarbonate & protons). CA has a zinc ion in its active site. The main function of CA is to keep acid-base balance in the blood stream and various tissues. This enzyme also assists Carbonic Anhydrase I to move CO2 to and from tissues.

CA1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 281 amino acids (1-261) and having a molecular mass of 31.0 kDa.CA1 Humanis fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:CA1: Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Belongs to the alpha-carbonic anhydrase family.
UniProt Protein Details:

Protein type:EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: cytosol

Molecular Function:carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; one-carbon compound metabolic process

NCBI Summary:Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Code:P00915
NCBI GenInfo Identifier:115449
NCBI Gene ID:759
NCBI Accession:P00915.2
UniProt Related Accession:P00915
Molecular Weight:261
NCBI Full Name:Carbonic anhydrase 1
NCBI Synonym Full Names:carbonic anhydrase I
NCBI Official Symbol:CA1��
NCBI Official Synonym Symbols:CAB; CA-I; Car1; HEL-S-11��
NCBI Protein Information:carbonic anhydrase 1; carbonic anhydrase B; carbonate dehydratase I; epididymis secretory protein Li 11
UniProt Protein Name:Carbonic anhydrase 1
UniProt Synonym Protein Names:Carbonate dehydratase I; Carbonic anhydrase B; CAB; Carbonic anhydrase I; CA-I
Protein Family:Cation-transporting ATPase
UniProt Gene Name:CA1��
UniProt Entry Name:CAH1_HUMAN

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