Human CA1 Recombinant Protein (RPPB1455)
- SKU:
- RPPB1455
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P00915
- Research Area:
- Enzymes
Description
| Product Name: | Human CA1 Recombinant Protein |
| Product Code: | RPPB1455 |
| Size: | 25µg |
| Species: | Human |
| Target: | CA1 |
| Synonyms: | CA-1, CA1, CAI, CA-I, Carbonate dehydratase I, Carbonic anhydrase I, Carbonic anhydrase 1, Car1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The Carbonic Anhydrase-1 protein solution contains 20mM Tris-HCl, pH-8, 1mM DTT and 10% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F |
Carbonic anhydrase 1 is a zinc metalloenzyme that catalyses reversible hydration of CO2 (CO2 + H2O=HCO3- + H+). Carbonic anhydrase 1 is essential to many biological processes such as cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, salvia, and gastric acid. Carbonic anhydrase 1 is abundant in erythrocytes and an early marker for erythroid differentiation.
Recombinant Human Carbonic anhydrase 1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 281 amino acids (1-261 a.a) and having a molecular mass of 31 kDa. Carbonic anhydrase 1 is fused to a�20 amino acids His-Tag at N-terminus and purified by conventional chromatography techniques.
| UniProt Protein Function: | CA1: Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Belongs to the alpha-carbonic anhydrase family. |
| UniProt Protein Details: | Protein type:EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase Chromosomal Location of Human Ortholog: 8q21.2 Cellular Component: cytosol Molecular Function:carbonate dehydratase activity; zinc ion binding Biological Process: bicarbonate transport; one-carbon compound metabolic process |
| NCBI Summary: | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| UniProt Code: | P00915 |
| NCBI GenInfo Identifier: | 115449 |
| NCBI Gene ID: | 759 |
| NCBI Accession: | P00915.2 |
| UniProt Related Accession: | P00915 |
| Molecular Weight: | 261 |
| NCBI Full Name: | Carbonic anhydrase 1 |
| NCBI Synonym Full Names: | carbonic anhydrase I |
| NCBI Official Symbol: | CA1�� |
| NCBI Official Synonym Symbols: | CAB; CA-I; Car1; HEL-S-11�� |
| NCBI Protein Information: | carbonic anhydrase 1; carbonic anhydrase B; carbonate dehydratase I; epididymis secretory protein Li 11 |
| UniProt Protein Name: | Carbonic anhydrase 1 |
| UniProt Synonym Protein Names: | Carbonate dehydratase I; Carbonic anhydrase B; CAB; Carbonic anhydrase I; CA-I |
| Protein Family: | Cation-transporting ATPase |
| UniProt Gene Name: | CA1�� |
| UniProt Entry Name: | CAH1_HUMAN |
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