Human C4BP alpha (C4 Binding Protein Alpha) CLIA Kit
The Human C4BP Alpha (C4 Binding Protein Alpha) CLIA Kit is a powerful tool for quantifying levels of C4BP Alpha in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for your research needs.C4BP Alpha is a key player in the regulation of the complement system, a crucial part of the immune response that helps to identify and eliminate pathogens. Dysregulation of the complement system has been implicated in various inflammatory and autoimmune diseases, making C4BP Alpha a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its easy-to-use format and reliable performance, the Human C4BP Alpha CLIA Kit is an essential tool for researchers investigating the role of C4BP Alpha in health and disease. Unlock new insights into the immune system and inflammation with this innovative kit from Assay Genie.
Product Name:
Human C4BP alpha (C4 Binding Protein Alpha) CLIA Kit
SKU:
HUES00284
Target:
Human C4BP alpha (C4 Binding Protein Alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C4BPα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C4BPα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C4BPα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human C4BPα. You can calculate the concentration of Human C4BPα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
101-115
89-104
Average (%)
101
109
96
1:4
Range (%)
100-111
101-118
95-112
Average (%)
106
108
103
1:8
Range (%)
93-107
101-117
95-113
Average (%)
99
107
103
1:16
Range (%)
94-105
87-100
98-112
Average (%)
100
92
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
102
EDTA plasma (n=5)
95-111
102
Cell culture media (n=5)
97-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.93
2.32
7.95
0.89
2.23
8.51
Standard deviation
0.09
0.2
0.76
0.09
0.16
0.59
C V (%)
9.68
8.62
9.56
10.11
7.17
6.93
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human C4BPα concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C4BPα in samples. No significant cross-reactivity or interference between Human C4BPα and analogues was observed.
