The Complement Component 4 (C4) ELISA Kit is engineered for the precise and quantitative measurement of human C4 levels across various biological samples.
As a key component of the complement system, C4 plays a crucial role in innate immunity, inflammation, and the clearance of immune complexes. Monitoring C4 levels is essential for assessing complement activation, immune system function, and autoimmune diseases. Accurate quantification of C4 aids in understanding its involvement in immune responses and inflammatory pathways. Our C4 ELISA Kit guarantees exceptional sensitivity and specificity, enabling the accurate and reproducible quantitation of C4 in biological samples. With stringent quality controls in place, this kit ensures robust performance and user-friendly protocols, catering to both research and clinical applications. Rely on Assay Genie's C4 ELISA Kit for reliable and precise quantification of this pivotal complement component to advance your research and diagnostic investigations.
Product Name:
Human C4 (Complement Component 4) ELISA Kit
SKU:
AEES00063
Target:
Human C4 (Complement Component 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C4. You can calculate the concentration of Human C4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
93-102
86-95
Average (%)
99
98
92
1:4
Range (%)
88-96
98-107
86-99
Average (%)
93
102
91
1:8
Range (%)
93-107
86-101
88-99
Average (%)
101
93
94
1:16
Range (%)
97-107
90-103
99-106
Average (%)
103
95
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
98
EDTA plasma (n=5)
100-110
104
Cell culture media (n=5)
96-105
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.76
23.42
88.0
9.77
23.56
82.34
Standard deviation
0.5
1.07
3.92
0.54
1.16
3.57
C V (%)
5.12
4.57
4.45
5.53
4.92
4.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human C4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C4 in samples. No significant cross-reactivity or interference between Human C4 and analogues was observed.
