The Human C3b Complement Fragment 3b ELISA Kit is a reliable and accurate tool for the detection of C3b complement fragment 3b levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides consistent and reproducible results, making it suitable for a variety of research applications.C3b complement fragment 3b is a key component of the complement system, playing a crucial role in immune response and inflammation. Its dysregulation has been implicated in various autoimmune diseases, infections, and inflammatory conditions, making it a valuable biomarker for studying and understanding these diseases.
This ELISA kit offers researchers a convenient and efficient way to measure C3b complement fragment 3b levels, allowing for in-depth investigations into its role in disease pathogenesis and potential therapeutic interventions. With its user-friendly protocol and reliable performance, the Human C3b Complement Fragment 3b ELISA Kit is an essential tool for any laboratory studying the complement system and its implications in human health.
Product Name:
Human C3b (Complement Fragment 3b) ELISA Kit
SKU:
HUES01956
Target:
Human C3b (Complement Fragment 3b)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C3b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C3b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C3b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C3b. You can calculate the concentration of Human C3b in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
93-107
95-108
Average (%)
104
100
101
1:4
Range (%)
88-103
87-99
88-101
Average (%)
95
92
94
1:8
Range (%)
87-102
81-92
88-100
Average (%)
94
87
93
1:16
Range (%)
89-104
84-98
83-97
Average (%)
96
91
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
94
EDTA plasma (n=5)
94-107
99
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
2.76
7.45
0.98
2.6
6.89
Standard deviation
0.06
0.16
0.35
0.05
0.15
0.31
C V (%)
5.5
5.8
4.7
5.1
5.77
4.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human C3b concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C3b in samples. No significant cross-reactivity or interference between Human C3b and analogues was observed.
