The Human C3 (Complement Component 3) ELISA Kit is a cutting-edge assay meticulously tailored for the precise quantitative measurement of Complement Component 3 levels in diverse biological samples.
C3 is a pivotal component of the complement system, essential for immune response modulation, inflammation regulation, and pathogen defense. As a key player in opsonization, chemotaxis, and the formation of the membrane-attack complex, C3 is integral to both innate and adaptive immune processes. This ELISA kit empowers researchers to elucidate the role of C3 in immune surveillance, inflammatory pathways, and various diseases. Accurate quantification of C3 is vital for unraveling its functions in immunity, autoimmune disorders, and infectious conditions. The C3 ELISA Kit provided by Assay Genie ensures exceptional sensitivity and specificity, guaranteeing precise and reproducible results. Crafted with stringent quality control measures, this kit delivers robust and reliable performance while remaining user-friendly. With Assay Genie's C3 ELISA Kit, researchers can confidently explore the intricacies of C3 involvement in immune responses and disease pathogenesis.
Product Name:
Human C3 (Complement Component 3) ELISA Kit
SKU:
AEES00090
Target:
Human C3 (Complement Component 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C3. You can calculate the concentration of Human C3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
90-100
97-111
Average (%)
101
95
104
1:4
Range (%)
92-103
90-97
92-106
Average (%)
97
93
100
1:8
Range (%)
91-98
89-104
93-109
Average (%)
94
96
101
1:16
Range (%)
97-110
98-105
89-100
Average (%)
105
102
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-109
104
EDTA plasma (n=5)
101-112
104
Cell culture media (n=5)
94-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.34
10.25
48.28
4.39
10.39
47.72
Standard deviation
0.23
0.48
2.33
0.24
0.48
1.96
C V (%)
5.3
4.68
4.83
5.47
4.62
4.11
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human C3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C3 in samples. No significant cross-reactivity or interference between Human C3 and analogues was observed.
