Human C12ORF5 Recombinant Protein (RPPB2927)
- SKU:
- RPPB2927
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9NQ88
Frequently bought together:
Description
| Product Name: | Human C12ORF5 Recombinant Protein |
| Product Code: | RPPB2927 |
| Size: | 10µg |
| Species: | Human |
| Target: | C12ORF5 |
| Synonyms: | Probable fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, TIGAR, C12orf5. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The C12ORF5 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 2mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMARFAL TVVRHGETRF NKEKIIQGQG VDEPLSETGF KQAAAAGIFL NNVKFTHAFS SDLMRTKQTM HGILERSKFC KDMTVKYDSR LRERKYGVVE GKALSELRAM AKAAREECPV FTPPGGETLD QVKMRGIDFF EFLCQLILKE ADQKEQFSQG SPSNCLETSL AEIFPLGKNH SSKVNSDSGI PGLAASVLVV SHGAYMRSLF DYFLTDLKCS LPATLSRSEL MSVTPNTGMS LFIINFEEGR EVKPTVQCIC MNLQDHLNGL TETR |
TP53-induced glycolysis and apoptosis regulator (TIGAR or C12ORF5), is a 270 amino acid protein induced by the p53 tumor suppressor pathway that functions to protect against oxidative stress. C12ORF5 specifically functions to block glycolysis, leading the pathway to the pentose phosphate shunt and decreasing the intracellular concentration of reactive oxygen species. Therefore, it is thought that C12ORF5 may act to modulate the apoptotic response to p53, thus allowing cells to survive mild or transient stresses.
C12ORF5 Human Recombinant fused with a 24 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 294 amino acids (1-270 a.a.) and having a molecular mass of 32.6kDa. The C12ORF5 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TIGAR: Fructose-bisphosphatase hydrolyzing fructose-2,6- bisphosphate as well as fructose-1,6-bisphosphate. Inhibits glycolysis by reducing cellular levels of fructose-2,6- bisphosphate. May protect cells against reactive oxygen species and against apoptosis induced by tp53. Belongs to the phosphoglycerate mutase family.Protein type: EC 3.1.3.46; Phosphatase (non-protein)Chromosomal Location of Human Ortholog: 12p13.3Cellular Component: cytoplasm; cytosol; intracellular; mitochondrial outer membrane; nucleusMolecular Function: bisphosphoglycerate phosphatase activity; fructose-2,6-bisphosphate 2-phosphatase activity; protein bindingBiological Process: apoptosis; autophagy; dephosphorylation; fructose 1,6-bisphosphate metabolic process; fructose 2,6-bisphosphate metabolic process; gene expression; negative regulation of glycolysis; negative regulation of programmed cell death; positive regulation of DNA repair; regulation of pentose-phosphate shunt; response to DNA damage stimulus; response to gamma radiation; response to xenobiotic stimulus; transcription initiation from RNA polymerase II promoter |
| UniProt Protein Details: | |
| NCBI Summary: | This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NQ88 |
| NCBI GenInfo Identifier: | 74734311 |
| NCBI Gene ID: | 57103 |
| NCBI Accession: | Q9NQ88.1 |
| UniProt Secondary Accession: | Q9NQ88,B2R840 |
| UniProt Related Accession: | Q9NQ88 |
| Molecular Weight: | 30,063 Da |
| NCBI Full Name: | Fructose-2,6-bisphosphatase TIGAR |
| NCBI Synonym Full Names: | TP53 induced glycolysis regulatory phosphatase |
| NCBI Official Symbol: | TIGAR�� |
| NCBI Official Synonym Symbols: | FR2BP; C12orf5�� |
| NCBI Protein Information: | fructose-2,6-bisphosphatase TIGAR |
| UniProt Protein Name: | Fructose-2,6-bisphosphatase TIGAR |
| UniProt Synonym Protein Names: | TP53-induced glycolysis and apoptosis regulator |
| Protein Family: | Fructose-2,6-bisphosphatase |
| UniProt Gene Name: | TIGAR�� |
| UniProt Entry Name: | TIGAR_HUMAN |
