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Human C-Type Lectin Domain Family 2, Member C (CLEC2C) ELISA Kit

SKU:
HUDL00593
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q07108
Sensitivity:
27pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD69,CLEC2-C,AIM,p60,EA1,MLR-3,Early T-Cell Activation Antigen p60,Early activation antigen CD69,Activation inducer molecule,Leukocyte surface antigen Leu-23
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human C-Type Lectin Domain Family 2, Member C (CLEC2C) ELISA Kit

The Human CLEC2C ELISA Kit is a powerful tool for measuring levels of C-type lectin domain family 2 member C (CLEC2C) in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and dependable results for a variety of research applications.CLEC2C is a member of the C-type lectin domain family, known for its role in immune responses and cell signaling. Research has shown that dysregulation of CLEC2C can be associated with autoimmune disorders, infectious diseases, and inflammatory conditions.

By measuring CLEC2C levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the Human CLEC2C ELISA Kit is a valuable tool for studying the role of CLEC2C in human health and disease, providing researchers with the means to explore new avenues for diagnosis and treatment.

Product Name:Human C-Type Lectin Domain Family 2, Member C (CLEC2C) ELISA Kit
Product Code:HUDL00593
Size:96 Assays
Target:CLEC2C
Synonyms:CD69, CLEC2-C, AIM, p60, EA1, MLR-3, Early T-Cell Activation Antigen p60, Early activation antigen CD69, Activation inducer molecule, Leukocyte surface antigen Leu-23
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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