The C-Peptide (C-P) ELISA Kit is meticulously designed for the quantitative measurement of C-Peptide levels in various human samples. C-peptide, an essential molecule produced when proinsulin is converted to insulin, serves as a marker for monitoring endogenous insulin secretion. Accurate quantification of C-peptide aids in assessing pancreatic beta cell function and insulin production in individuals, providing valuable insights into diabetes management and related metabolic disorders.
This ELISA kit ensures exceptional sensitivity and specificity, delivering precise and reproducible results. With stringent quality control measures in place, this kit offers robust performance and user-friendly protocols, making it an excellent choice for both research and clinical applications. Trust Assay Genie's C-Peptide ELISA Kit for accurate and reliable quantification of this pivotal biomarker in your studies.
Product Name:
Human C-P (C-Peptide) ELISA Kit
SKU:
AEES00056
Target:
Human C-P (C-Peptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C-P. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C-P and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C-P, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C-P. You can calculate the concentration of Human C-P in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-110
90-98
92-100
Average (%)
103
95
97
1:4
Range (%)
86-99
96-104
96-103
Average (%)
94
101
99
1:8
Range (%)
85-96
91-102
95-106
Average (%)
91
98
103
1:16
Range (%)
100-113
89-99
85-97
Average (%)
105
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-113
105
EDTA plasma (n=5)
87-96
92
Cell culture media (n=5)
97-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24
56.7
249.89
22.74
53.34
200.91
Standard deviation
1.33
2.65
11.0
1.27
2.41
10.75
C V (%)
5.54
4.67
4.4
5.58
4.52
5.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human C-P concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C-P in samples. No significant cross-reactivity or interference between Human C-P and analogues was observed.
