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Human BTK ELISA Kit

SKU:
HUFI01677
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q06187
Sensitivity:
0.094ng/ml
Range:
0.156-10ng/ml
ELISA Type:
Sandwich
Synonyms:
BTK, Tyrosine-protein kinase BTK, Bruton tyrosine kinase, Agammaglobulinaemia tyrosine kinase, ATK, B-cell progenitor kinase, BPK, AT, XLA, IMD1, AGMX1, PSCTK1, dominant-negative kinase-deficient Brutons tyrosine kinase, Bruton agammaglobulinemia tyr
Reactivity:
Human
Research Area:
Immunology
$719
Frequently bought together:

Description

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Human BTK ELISA Kit

The Human BTK (Bruton's Tyrosine Kinase) ELISA Kit is a powerful tool for the precise measurement of BTK levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers reliable and consistent results, making it perfect for various research applications.BTK is a key enzyme in the B-cell receptor signaling pathway, playing a crucial role in immune response regulation. Dysregulation of BTK has been linked to various immunological disorders, including autoimmune diseases and certain types of cancers.

By accurately measuring BTK levels, researchers can gain valuable insights into these conditions and potentially uncover new therapeutic targets.Don't miss out on the opportunity to advance your research with the Human BTK ELISA Kit. Order yours today and unlock the potential of studying BTK in human samples with precision and accuracy.

Product Name:Human BTK ELISA Kit
Product Code:HUFI01677
Size:96 Assays
Alias:BTK, Tyrosine-protein kinase BTK, Bruton tyrosine kinase, Agammaglobulinaemia tyrosine kinase, ATK, B-cell progenitor kinase, BPK, AT, XLA, IMD1, AGMX1, PSCTK1, dominant-negative kinase-deficient Brutons tyrosine kinase, Bruton agammaglobulinemia tyrosine kinase
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human BTK concentrations in serum plasma and other biological fluids.
Sensitivity:0.094ng/ml
Range:0.156-10ng/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human BTK and the recovery rates were calculated by comparing the measured value to the expected amount of Human BTK in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)90-10295
EDTA plasma(n=5)86-10093
UFH plasma(n=5)87-10493
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human BTK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)95-105%87-97%86-103%
EDTA plasma(n=5)88-100%82-97%82-100%
UFH plasma(n=5)82-91%84-98%80-100%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotQ06187
UniProt Protein Function:Btk: a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis.
UniProt Protein Details:Protein type:Kinase, protein; Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Tec family

Cellular Component: cytoplasm; cytoplasmic vesicle; cytosol; extrinsic to internal side of plasma membrane; intracellular membrane-bound organelle; lipid raft; mast cell granule; membrane; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function:ATP binding; identical protein binding; kinase activity; lipid binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; phosphatidylinositol-3,4,5-triphosphate binding; protein binding; protein kinase activity; protein-tyrosine kinase activity; transferase activity

Biological Process: adaptive immune response; apoptosis; cell maturation; histamine secretion by mast cell; I-kappaB kinase/NF-kappaB cascade; immune system process; innate immune response; negative regulation of cytokine production; peptidyl-tyrosine phosphorylation; phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; regulation of transcription, DNA-dependent; response to organic substance; transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway

NCBI Summary:The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]
UniProt Code:Q06187
NCBI GenInfo Identifier:547759
NCBI Gene ID:695
NCBI Accession:Q06187.3
UniProt Secondary Accession:Q06187,Q61365,
UniProt Related Accession:Q06187
Molecular Weight:
NCBI Full Name:Tyrosine-protein kinase BTK
NCBI Synonym Full Names:Bruton tyrosine kinase
NCBI Official Symbol:BTK  
NCBI Official Synonym Symbols:AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1  
NCBI Protein Information:tyrosine-protein kinase BTK
UniProt Protein Name:Tyrosine-protein kinase BTK
UniProt Synonym Protein Names:Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase; Kinase EMB
Protein Family:Tyrosine-protein kinase
UniProt Gene Name:Btk  
UniProt Entry Name:BTK_MOUSE

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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