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Human BRMS1 Recombinant Protein (RPPB2909)

SKU:
RPPB2909
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9HCU9
$371
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Description

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Product Name:Human BRMS1 Recombinant Protein
Product Code:RPPB2909
Size:10µg
Species:Human
Target:BRMS1
Synonyms:Breast Cancer Metastasis Suppressor 1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The BRMS1 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 80% as determined by SDS-PAGE.
Amino Acid Sequence:MASMTGGQQM GRGSHMPVQP PSKDTEEMEA EGDSAAEMNG EEEESEEERS GSQTESEEES SEMDDEDYER RRSECVSEML DLEKQFSELK EKLFRERLSQ LRLRLEEVGA ERAPEYTEPL GGLQRSLKIR IQVAGIYKGF CLDVIRNKYE CELQGAKQHL ESEKLLLYDT LQGELQERIQ RLEEDRQSLD LSSEWWDDKL HARGSSRSWD SLPPSKRKKA PLVSGPYIVY MLQEIDILED WTAIKKARAA VSPQKRKSDG P

BRMS1 protein, a member of the mSin3a family of HDAC (histone deacetylase complexes), is mainly restricted to the nucleus. BRMS1 holds two coiled-coil motifs and a few imperfect leucine zipper motifs. BRMS1 reduces the metastatic potential, but not the tumorogenicity, of melanoma cell lines and human breast cancer. Alternative splicing creates two transcript variants encoding different isoforms.

BRMS1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 261 amino acids (1-246) and having a molecular mass of 29.9 kDa. BRMS1 is fused to a 15 amino acid T7-tag at N-terminus.

UniProt Protein Function:BRMS1: Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down- regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma. Belongs to the BRMS1 family.
UniProt Protein Details:

Protein type:Tumor suppressor

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function:histone deacetylase activity; histone deacetylase binding; NF-kappaB binding; protein binding

Biological Process: histone deacetylation; inhibition of NF-kappaB transcription factor; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent

NCBI Summary:This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:Q9HCU9
NCBI GenInfo Identifier:18202959
NCBI Gene ID:25855
NCBI Accession:Q9HCU9.1
UniProt Secondary Accession:Q9HCU9,Q6IAI2,
UniProt Related Accession:Q9HCU9
Molecular Weight:28,461 Da
NCBI Full Name:Breast cancer metastasis-suppressor 1
NCBI Synonym Full Names:breast cancer metastasis suppressor 1
NCBI Official Symbol:BRMS1��
NCBI Protein Information:breast cancer metastasis-suppressor 1
UniProt Protein Name:Breast cancer metastasis-suppressor 1
Protein Family:Breast cancer metastasis-suppressor
UniProt Gene Name:BRMS1��
UniProt Entry Name:BRMS1_HUMAN

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