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Human Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit

SKU:
HUDL00355
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P51587
Sensitivity:
0.107ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
BRCA2,BRCC2,FACD,FAD,FAD1,FANCD,FANCD1,Breast Cancer 2,Early Onset,Breast Cancer Type 2 Susceptibility Protein,Fanconi anemia group D1 protein
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit

The Human Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit is a cutting-edge tool for the precise and reliable measurement of BRCA2 levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit offers superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.BRCA2 is a key protein involved in DNA repair and maintenance, particularly in the context of breast cancer susceptibility. Mutations in the BRCA2 gene are associated with an increased risk of hereditary breast and ovarian cancers, highlighting the importance of studying this biomarker for early detection and personalized treatment strategies.

With the BRCA2 ELISA Kit, researchers can streamline their efforts to understand the molecular mechanisms underlying breast cancer and other related diseases. This comprehensive kit provides a valuable tool for investigating BRCA2 function, disease pathology, and potential therapeutic targets in the quest for improved patient outcomes.

Product Name:Human Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit
Product Code:HUDL00355
Size:96 Assays
Target:BRCA2
Synonyms:BRCA2, BRCC2, FACD, FAD, FAD1, FANCD, FANCD1, Breast Cancer 2,Early Onset, Breast Cancer Type 2 Susceptibility Protein, Fanconi anemia group D1 protein
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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