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Human BRAF ELISA Kit

SKU:
AEES00666
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Human
Sensitivity:
0.19 ng/mL
Range:
0.31-20 ng/mL
Sample Type:
Serum, plasma and other biological fluids
$599
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Description

Human BRAF ELISA Kit

The Human BRAF ELISA Kit is an advanced assay meticulously designed for the precise and quantitative measurement of BRAF protein levels in human biological samples. BRAF, a critical gene encoding a serine/threonine protein kinase, plays a pivotal role in cell signaling pathways that regulate cell growth, proliferation, and differentiation. This ELISA kit offers a cutting-edge solution for researchers and clinicians to explore BRAF expression levels and understand its significance in cellular signaling cascades. Researchers utilizing the Human BRAF ELISA Kit can accurately quantify BRAF protein levels, providing valuable insights into its involvement in various cellular processes and signal transduction pathways.

With exceptional sensitivity and specificity, this kit ensures accurate and reproducible results, facilitating detailed studies on BRAF-mediated signaling mechanisms and its implications in physiological and pathological conditions. Manufactured under rigorous quality control measures, the Human BRAF ELISA Kit offers robust performance and user-friendly operation, making it an indispensable tool for investigations related to BRAF signaling, oncogenesis, and targeted therapy development. Explore the intricacies of BRAF-mediated signaling pathways with confidence using this high-quality ELISA kit.

Product Name:Human BRAF ELISA Kit
Product Code:AEES00666
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Human
Detection Range:0.31-20 ng/mL
Sensitivity:0.19 ng/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Human BRAF in samples. No significant cross-reactivity or interference between Human BRAF and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Human BRAF concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BRAF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BRAF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BRAF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BRAF. You can calculate the concentration of Human BRAF in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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