Human BMPR2 (Bone Morphogenetic Protein Receptor II) ELISA Kit
The Human BMPR2 (Bone Morphogenetic Protein Receptor II) ELISA Kit is specifically designed for the precise measurement of BMPR2 levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an excellent tool for various research applications.BMPR2 is a vital receptor involved in the BMP signaling pathway, playing a crucial role in regulating cell differentiation and growth. Dysregulation of BMPR2 has been linked to various diseases, including pulmonary arterial hypertension and certain types of cancer, highlighting its significance as a biomarker for disease research and drug development.
By using the Human BMPR2 ELISA Kit, researchers can gain valuable insights into the role of BMPR2 in disease pathogenesis and potentially uncover new therapeutic targets. This kit offers a reliable and efficient way to study BMPR2 levels, paving the way for advancements in the field of biomedical research.
Product Name:
Human BMPR2 (Bone Morphogenetic Protein Receptor II) ELISA Kit
SKU:
HUES01763
Target:
Human BMPR2 (Bone Morphogenetic Protein Receptor II)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BMPR2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BMPR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMPR2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BMPR2. You can calculate the concentration of Human BMPR2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
97-108
87-101
Average (%)
103
103
94
1:4
Range (%)
92-105
81-96
86-99
Average (%)
99
87
91
1:8
Range (%)
87-100
83-98
89-101
Average (%)
93
89
94
1:16
Range (%)
89-103
80-90
87-101
Average (%)
95
85
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
90
EDTA plasma (n=5)
88-103
95
Cell culture media (n=5)
87-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.1
3.37
0.56
1.11
3.14
Standard deviation
0.04
0.05
0.12
0.03
0.05
0.14
C V (%)
7.55
4.55
3.56
5.36
4.5
4.46
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human BMPR2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BMPR2 in samples. No significant cross-reactivity or interference between Human BMPR2 and analogues was observed.
