Human BMP-6 (Bone Morphogenetic Protein 6) CLIA Kit
The Human BMP-6 (Bone Morphogenetic Protein-6) CLIA Kit is a highly reliable and accurate tool for detecting BMP-6 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications. BMP-6 is a key protein involved in bone formation and regeneration, as well as other critical functions such as cell differentiation and tissue development. Dysregulation of BMP-6 has been linked to various disorders, including skeletal diseases, inflammation, and cancer.
Therefore, this CLIA kit is essential for studying these conditions and potentially developing targeted treatments. With this advanced kit, researchers can gain valuable insights into the role of BMP-6 in various physiological and pathological processes, ultimately advancing our understanding of human health and disease. Trust the Human BMP-6 CLIA Kit for accurate quantification and analysis of this important biomarker. Visit the provided URL for more information.
Product Name:
Human BMP-6 (Bone Morphogenetic Protein 6) CLIA Kit
SKU:
HUES00396
Target:
Human BMP-6 (Bone Morphogenetic Protein 6)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BMP-6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BMP-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMP-6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BMP-6. You can calculate the concentration of Human BMP-6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
100-115
102-118
Average (%)
97
108
110
1:4
Range (%)
96-110
102-118
99-117
Average (%)
102
108
107
1:8
Range (%)
86-100
93-109
88-101
Average (%)
93
100
94
1:16
Range (%)
84-98
90-105
98-112
Average (%)
90
96
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-112
103
EDTA plasma (n=5)
96-111
103
Cell culture media (n=5)
94-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.83
311.65
678.7
93.24
304.21
679.48
Standard deviation
10.39
25.09
59.32
11.43
30.97
60.81
C V (%)
11.31
8.05
8.74
12.26
10.18
8.95
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BMP-6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BMP-6 in samples. No significant cross-reactivity or interference between Human BMP-6 and analogues was observed.
