Human BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit
The Human BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit is a reliable and accurate tool for the measurement of BMP-2 levels in human samples including serum, plasma, and cell culture supernatants. This kit is designed with high sensitivity and specificity, ensuring consistent and precise results for various research applications.BMP-2 is a key protein involved in bone and cartilage formation, playing a crucial role in skeletal development and regeneration.
Dysregulation of BMP-2 has been implicated in various musculoskeletal disorders, making it a valuable biomarker for understanding these conditions and developing therapeutic interventions.With the Human BMP-2 ELISA Kit, researchers can confidently study the role of BMP-2 in bone health, osteoporosis, and other related diseases, leading to advancements in the field of regenerative medicine and personalized treatment strategies.
Product Name:
Human BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit
SKU:
HUES01298
Target:
Human BMP-2 (Bone Morphogenetic Protein 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BMP-2. You can calculate the concentration of Human BMP-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
86-102
97-108
Average (%)
92
93
103
1:4
Range (%)
97-112
80-94
91-104
Average (%)
105
87
98
1:8
Range (%)
98-114
81-94
91-108
Average (%)
106
86
98
1:16
Range (%)
94-110
83-94
96-106
Average (%)
100
89
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-97
92
EDTA plasma (n=5)
84-97
91
Cell culture media (n=5)
85-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
199.9
617.2
1476.0
215.6
594.5
1427.3
Standard deviation
10.4
25.9
45.8
11.0
25.0
47.1
C V (%)
5.2
4.2
3.1
5.1
4.21
3.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human BMP-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BMP-2 in samples. No significant cross-reactivity or interference between Human BMP-2 and analogues was observed.
