Human BMP-1 (Bone Morphogenetic Protein 1) CLIA Kit
The Human BMP-1 (Bone Morphogenetic Protein-1) CLIA Kit is a cutting-edge tool designed for the precise measurement of BMP-1 levels in human samples including serum, plasma, and cell culture supernatants. With a focus on high sensitivity and specificity, this kit delivers accurate and consistent results, making it a valuable asset for a diverse array of research projects.BMP-1 is a key player in the regulation of bone and tissue formation, with implications in various diseases and disorders.
Its role in processes such as cell differentiation and tissue repair underscores its importance as a biomarker for studying conditions like osteoporosis, arthritis, and skeletal disorders. This CLIA kit offers researchers a reliable means of exploring the biological mechanisms involving BMP-1 and its potential therapeutic applications.
Product Name:
Human BMP-1 (Bone Morphogenetic Protein 1) CLIA Kit
SKU:
HUES00393
Target:
Human BMP-1 (Bone Morphogenetic Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BMP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BMP-1. You can calculate the concentration of Human BMP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-102
97-115
100-114
Average (%)
94
105
107
1:4
Range (%)
103-118
90-100
98-113
Average (%)
109
95
105
1:8
Range (%)
87-102
88-103
93-107
Average (%)
94
94
98
1:16
Range (%)
88-100
91-107
89-104
Average (%)
95
99
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
97
EDTA plasma (n=5)
96-112
103
Cell culture media (n=5)
101-116
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.48
227.71
960.15
103.31
218.77
908.62
Standard deviation
11.69
16.67
78.16
12.76
17.9
69.15
C V (%)
11.87
7.32
8.14
12.35
8.18
7.61
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BMP-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BMP-1 in samples. No significant cross-reactivity or interference between Human BMP-1 and analogues was observed.
