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Human BH3 Interacting Domain Death Agonist (Bid) ELISA Kit

SKU:
HUDL00327
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P55957
Sensitivity:
0.115ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
p22 BID
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Human BH3 Interacting Domain Death Agonist (Bid) ELISA Kit

The Human BH3 Interacting Domain Death Agonist (BID) ELISA Kit is a reliable tool for the detection of BID levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it suitable for a variety of research applications.BID is a key protein involved in apoptosis, playing a critical role in regulating cell death pathways. Dysregulation of BID has been linked to various diseases such as cancer, neurodegenerative disorders, and autoimmune conditions, making it a valuable biomarker for understanding the mechanisms underlying these conditions and exploring potential therapeutic interventions.

Overall, the Human BID ELISA Kit offers researchers a powerful tool for studying the role of BID in health and disease, with its precision and reliability supporting valuable insights in the field of molecular biology and medicine.

Product Name:Human BH3 Interacting Domain Death Agonist (Bid) ELISA Kit
Product Code:HUDL00327
Size:96 Assays
Target:Bid
Synonyms:p22 BID
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)86-10093
EDTA plasma (n=5)87-10093
Heparin plasma (n=5)88-10094
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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