The Human BGN (Biglycan) ELISA Kit is expertly designed to facilitate the precise quantitative measurement of Biglycan in various human biological samples.
Biglycan, a critical component of the extracellular matrix, plays a vital role in tissue integrity, cellular signaling, and modulation of immune responses. As a key regulator of inflammatory processes and matrix organization, accurate assessment of Biglycan levels is essential in understanding its impact on tissue homeostasis and disease pathology. The Assay Genie's BGN ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for your research and clinical studies. Manufactured under strict quality control standards, this kit delivers reliable performance and user-friendly protocols, making it a valuable tool for investigating the roles of Biglycan in various physiological and pathological processes. Trust Assay Genie's BGN ELISA Kit for precise and dependable quantification of this important biomarker in your scientific investigations.
Product Name:
Human BGN (Biglycan) ELISA Kit
SKU:
AEES00125
Target:
Human BGN (Biglycan)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BGN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BGN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BGN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BGN. You can calculate the concentration of Human BGN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
84-98
99-107
Average (%)
90
91
104
1:4
Range (%)
90-103
86-99
94-104
Average (%)
95
91
99
1:8
Range (%)
87-103
93-101
89-97
Average (%)
95
97
93
1:16
Range (%)
85-98
96-105
102-113
Average (%)
93
101
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-103
98
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
97-106
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
86.42
231.29
870.65
93.68
217.27
864.49
Standard deviation
4.37
11.47
36.48
5.01
9.84
39.77
C V (%)
5.06
4.96
4.19
5.35
4.53
4.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human BGN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BGN in samples. No significant cross-reactivity or interference between Human BGN and analogues was observed.
