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Human Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit

SKU:
HUDL00303
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P56817
Sensitivity:
0.11ng/mL
Range:
0.25-16ng/mL
ELISA Type:
Sandwich
Synonyms:
ASP2,Beta Secretase,Memapsin 2,Aspartyl protease 2,Beta-site amyloid precursor protein cleaving enzyme 1,Membrane-associated aspartic protease 2
Reactivity:
Human
Sample Type:
Serum, Plasma, Cerebrospinal Fluid And Other Biological Fluids
€649
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Description

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Human Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit

The Human Beta-site APP Cleaving Enzyme 1 (BACE1) ELISA Kit is specifically designed for the sensitive and accurate detection of BACE1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.BACE1 is a key enzyme involved in the processing of amyloid precursor protein (APP), leading to the production of amyloid beta peptides that are implicated in the development of Alzheimer's disease.

Monitoring BACE1 levels can provide valuable insights into the pathogenesis of Alzheimer's disease and potential therapeutic strategies.With its advanced technology and optimized protocols, the Human BACE1 ELISA Kit is a valuable tool for researchers investigating neurodegenerative disorders and developing novel treatments. Trust in the accuracy and precision of this ELISA kit to advance your research in this critical area of study.

Product Name:Human Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit
Product Code:HUDL00303
Size:96 Assays
Target:bACE1
Synonyms:ASP2, Beta Secretase, Memapsin 2, Aspartyl protease 2, Beta-site amyloid precursor protein cleaving enzyme 1, Membrane-associated aspartic protease 2
Detection Method:Sandwich
Range:0.25-16ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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