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Human Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1) ELISA Kit

SKU:
HUDL00320
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9HAY6
Sensitivity:
0.103ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
BCO1,BCDO,BCDO1,BCMO,Beta-carotene dioxygenase 1,Beta-carotene oxygenase 1,Beta,beta-carotene 15,15'-monooxygenase
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1) ELISA Kit

The Human Beta-carotene 15,15'-monooxygenase 1 (BCMO1) ELISA Kit is specifically designed for the accurate detection of BCMO1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.BCMO1 is an important enzyme involved in the conversion of dietary beta-carotene into vitamin A, an essential nutrient for vision, immune function, and overall health.

Deficiencies in BCMO1 have been linked to various health issues, making it a key biomarker for studying nutrient metabolism and potential therapeutic interventions.With its reliable performance and ease of use, the Human BCMO1 ELISA Kit is a valuable tool for researchers investigating vitamin A metabolism, nutritional deficiencies, and related health conditions. Get accurate and reliable results with this advanced ELISA kit from Assay Genie.

Product Name:Human Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1) ELISA Kit
Product Code:HUDL00320
Size:96 Assays
Target:bCMO1
Synonyms:BCO1, BCDO, BCDO1, BCMO, Beta-carotene dioxygenase 1, Beta-carotene oxygenase 1, Beta,beta-carotene 15,15'-monooxygenase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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