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Human BECN1 Recombinant Protein (RPPB2882)

SKU:
RPPB2882
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q14457
$371
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Description

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Product Name:Human BECN1 Recombinant Protein
Product Code:RPPB2882
Size:10µg
Species:Human
Target:BECN1
Synonyms:ATG6, beclin1, VPS30, Coiled-coil myosin-like BCL2-interacting protein, Beclin-1, GT197.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:BECN1 protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.5), 0.1M NaCl, 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MEGSKTSNNS TMQVSFVCQR CSQPLKLDTS FKILDRVTIQ ELTAPLLTTA QAKPGETQEE ETNSGEEPFI ETPRQDGVSR RFIPPARMMS TESANSFTLI GEASDGGTME NLSRRLKVTG DLFDIMSGQT DVDHPLCEEC TDTLLDQLDT QLNVTENECQ NYKRCLEILE QMNEDDSEQL QMELKELALE EERLIQELED VEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQQ LELDDELKSV ENQMRYAQTQ LDKLKKTNVF NATFHIWHSG QFGTINNFRL GRLPSVPVEW NEINAAWGQT VLLLHALANK MGLKFQRYRL VPYGNHSYLE SLTDKSKELP LYCSGGLRFF WDNKFDHAMV AFLDCVQQFK EEVEKGETRF CLPYRMDVEK GKIEDTGGSG GSYSIKTQFN SEEQWTKALK FMLTNLKWGL AWVSSQFYNK

BECN1, Beclin-1 and autophagy-related gene (Atg) 6, is a member of the beclin family. BECN1 has a key role in autophagy and antiviral host defense. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are vital for the initiation of the formation of the autophagasome in autophagy. Also it is required for the abscission step of cytokinesis.

BECN1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 470 amino acids (1-450 a.a) and having a molecular mass of 54.1kDa.BECN1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:beclin 1: an essential autophagy protein that has been linked to multiple processes including tumor suppression, protection against some cardiac and neurological degenerative diseases, and lifespan extension. A ubiquitous protein of the beclin family. Is part of the class III PI3K complex that induces autophagy. Expressed at elevated levels in dendrites and cell bodies of cerebellar Purkinje cells. Interacts with the anti-apoptotic protein Bcl-2, which inhibits autophagy when it is present in the endoplasmic reticulum. The dissociation of ATG6 from Bcl-2 is essential for its autophagic activity. Interacts with PITC and GluR-delta2. Probably forms a complex with AMBRA1 and PIK3C3. Plays a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
UniProt Protein Details:

Protein type:Adaptor/scaffold; Autophagy; Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: extrinsic to membrane; mitochondrion; endoplasmic reticulum; cytoplasmic membrane-bound vesicle; dendrite; phagocytic vesicle; trans-Golgi network; nucleus; endosome

Molecular Function:protein binding; phosphoinositide 3-kinase binding

Biological Process: response to drug; viral reproduction; late endosome to vacuole transport; cytokinesis; CVT pathway; cellular response to nitrogen starvation; negative regulation of cell proliferation; response to vitamin E; lysosome organization and biogenesis; regulation of catalytic activity; beta-amyloid metabolic process; mitochondrion degradation; response to hypoxia; cellular defense response; neuron development; defense response to virus; positive regulation of macroautophagy; positive regulation of autophagy; negative regulation of apoptosis; autophagic vacuole formation

NCBI Summary:Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM, Jul 2010]
UniProt Code:Q14457
NCBI GenInfo Identifier:4680381
NCBI Gene ID:8678
NCBI Accession:AAD27650.1
UniProt Secondary Accession:Q14457,O75595, Q9UNA8,
UniProt Related Accession:Q14457
Molecular Weight:51,896 Da
NCBI Full Name:beclin 1
NCBI Synonym Full Names:beclin 1, autophagy related
NCBI Official Symbol:BECN1��
NCBI Official Synonym Symbols:ATG6; VPS30; beclin1��
NCBI Protein Information:beclin-1; ATG6 autophagy related 6 homolog; coiled-coil myosin-like BCL2-interacting protein; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
UniProt Protein Name:Beclin-1
UniProt Synonym Protein Names:Coiled-coil myosin-like BCL2-interacting protein; Protein GT197
Protein Family:Beclin
UniProt Gene Name:BECN1��
UniProt Entry Name:BECN1_HUMAN

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