The Human BECN1 (Beclin-1) CLIA Kit offered by Assay Genie is specifically designed for the accurate detection of Beclin-1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.Beclin-1 is a key player in the regulation of autophagy, a cellular process that plays a critical role in maintaining cellular homeostasis and responding to stress conditions. Dysregulation of autophagy and Beclin-1 has been implicated in various diseases, including cancer, neurodegenerative disorders, and infectious diseases.
By accurately measuring Beclin-1 levels, researchers can gain valuable insights into the autophagy pathway and its role in disease development and progression. This kit provides a valuable tool for studying the role of Beclin-1 in various physiological and pathological processes, ultimately leading to a better understanding of disease mechanisms and potential therapeutic targets.
Product Name:
Human BECN1 (Beclin 1) CLIA Kit
SKU:
HUES00385
Target:
Human BECN1 (Beclin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BECN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BECN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BECN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BECN1. You can calculate the concentration of Human BECN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
98-111
86-98
Average (%)
95
104
91
1:4
Range (%)
89-101
91-102
86-99
Average (%)
96
97
93
1:8
Range (%)
101-114
93-106
99-115
Average (%)
107
98
106
1:16
Range (%)
101-115
94-109
96-111
Average (%)
107
100
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
92
EDTA plasma (n=5)
94-109
100
Cell culture media (n=5)
97-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.33
195.23
825.53
98.45
195.77
789.61
Standard deviation
7.93
14.33
77.27
11.21
22.28
58.27
C V (%)
8.06
7.34
9.36
11.39
11.38
7.38
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BECN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BECN1 in samples. No significant cross-reactivity or interference between Human BECN1 and analogues was observed.
