null

Human Basic Salivary Proline Rich Protein 2 (PRB2) ELISA Kit

SKU:
HUDL02387
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P02812
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PRPPRB1,Ps,cP7,Proline-Rich Protein BstNI Subfamily 2,Con1 glycoprotein,Basic peptide P-F
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Basic Salivary Proline Rich Protein 2 (PRB2) ELISA Kit

The Human Basic Salivary Proline-Rich Protein 2 (PRB2) ELISA Kit is a cutting-edge tool designed for accurate detection of PRB2 levels in human saliva samples. This kit is highly sensitive and specific, ensuring precise and consistent results for research applications.PRB2 is an important protein found in saliva, with roles in antimicrobial defense and maintaining oral health. Its levels can be influenced by various factors such as stress, diet, and oral hygiene practices, making it a valuable biomarker for studying oral health conditions and their potential treatments.

With the Human Basic Salivary Proline-Rich Protein 2 (PRB2) ELISA Kit, researchers can confidently analyze and quantify PRB2 levels in human saliva samples, advancing our understanding of oral health and potentially leading to new therapeutic approaches.

Product Name:Human Basic Salivary Proline Rich Protein 2 (PRB2) ELISA Kit
Product Code:HUDL02387
Size:96 Assays
Target:PRB2
Synonyms:PRPPRB1, Ps, cP7, Proline-Rich Protein BstNI Subfamily 2, Con1 glycoprotein, Basic peptide P-F
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products