Human B-Lymphocyte Activation Antigen B7-2 (LAB7-2) ELISA Kit
The Human B Lymphocyte Activation Antigen B7-2 (LAB7-2) ELISA Kit is a powerful tool for investigating the expression levels of B7-2 in human samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and consistent results that are essential for a variety of research applications.B7-2, also known as CD86, is a critical co-stimulatory molecule involved in the activation of B lymphocytes and T lymphocytes. Its expression can significantly impact immune responses, making it a key player in conditions such as autoimmune diseases, infectious diseases, and cancer.
By accurately quantifying B7-2 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Whether studying immune system function, investigating disease pathways, or developing novel treatment strategies, the Human B Lymphocyte Activation Antigen B7-2 ELISA Kit provides the necessary tools for advanced research in immunology and beyond. Trust in its reliability and accuracy to drive your discoveries forward.
Product Name:
Human B-Lymphocyte Activation Antigen B7-2 (LAB7-2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
82-102
92
EDTA plasma (n=5)
90-101
95
Heparin plasma (n=5)
88-100
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
