Human Autophagy Array (20 targets) (SARB0023)
- SKU:
- SARB0023
- Product Type:
- Protein Array
- Analytes:
- ATG12
- ATG7
- ATG10
- ATG13
- ATG3
- ATG4A
- ATG4B
- ATG5
- Beclin
- BNIP3L
- DDR2
- GABARAP
- LAMP1 (CD107a)
- LC3a
- LC3B
- NBS1
- P62
- RHEB
- MSK1
- Alpha-synuclein
- Reactivity:
- Human
- Applications:
- Multiplex Array
Description
Human Autophagy Array (20 targets) (SARB0023)
The Human Autophagy Array - 20 Targets (SARB0023) is a powerful tool for researchers studying the process of autophagy, a critical cellular mechanism involved in maintaining cellular homeostasis and responding to stress. This array allows for the simultaneous detection and quantification of 20 key autophagy-related proteins, providing valuable insights into the regulation of this important cellular process.With a focus on key autophagy targets such as LC3, p62, Beclin-1, and ATG5, this array enables researchers to study the dynamic changes in protein expression levels associated with autophagy induction and progression. The array is designed for use with human samples, offering researchers a comprehensive overview of autophagy-related protein expression in various cell types and biological contexts.
Whether investigating the role of autophagy in cancer, neurodegenerative diseases, or metabolic disorders, the Human Autophagy Array - 20 Targets (SARB0023) provides a reliable and efficient platform for analyzing multiple autophagy-related proteins in a single experiment. By gaining a deeper understanding of the molecular mechanisms governing autophagy, researchers can identify novel therapeutic targets and develop potential treatments for a wide range of diseases.
| Product Sku: | SARB0023 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 20 |
| Gene Symbols: | ATG10, ATG12, ATG13, ATG3, ATG4A, ATG4B, ATG5, ATG7, BECN1, BNIP3L|DDR2, GABARAP, LAMP1, MAP1LC3A, MAP1LC3B, NBN, RHEB, RPS6KA5, SNCA, SQSTM1 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
ATG12
| ATG7
| ATG10
| ATG13
| ATG3
|
ATG4A
| ATG4B
| ATG5
| Beclin
| BNIP3L
|
DDR2
| GABARAP
| LAMP1 (CD107a)
| LC3a
| LC3B
|
NBS1
| P62
| RHEB
| MSK1
| Alpha-synuclein
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Autophagy Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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