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Human Aurora Kinase B (AURKB) ELISA Kit

SKU:
HUDL00294
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q96GD4
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
AIM1,AIK2,ARK2,AurB,IPL1,STK1,STK12,STK5,Serine/Threonine Kinase 12,Aurora-1,Aurora-and IPL1-like midbody-associated protein 1,Aurora/IPL1-related kinase 2
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Aurora Kinase B (AURKB) ELISA Kit

The Human Aurora Kinase B (AURKB) ELISA Kit is a powerful tool for the precise measurement of Aurora Kinase B levels in human biological samples. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results for a variety of research purposes.Aurora Kinase B is a key enzyme involved in cell division and plays a crucial role in ensuring the accurate segregation of chromosomes during cell division.

Dysregulation of Aurora Kinase B has been implicated in various diseases, including cancer, making it a valuable biomarker for understanding disease mechanisms and developing targeted therapies.With the Human Aurora Kinase B (AURKB) ELISA Kit, researchers can confidently measure Aurora Kinase B levels in serum, plasma, and cell culture supernatants, enabling advances in cancer research, drug development, and other fields of study.

Product Name:Human Aurora Kinase B (AURKB) ELISA Kit
Product Code:HUDL00294
Size:96 Assays
Target:AURKB
Synonyms:AIM1, AIK2, ARK2, AurB, IPL1, STK1, STK12, STK5, Serine/Threonine Kinase 12, Aurora-1, Aurora-and IPL1-like midbody-associated protein 1, Aurora/IPL1-related kinase 2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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