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Human Atrial Natriuretic Peptide (ANP) ELISA Kit

SKU:
HUDL00182
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P01160
Sensitivity:
5.7pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
ANH,ANF,CDD,Atrial Natriuretic Factor,Atrial Natriuretic Hormone,Atriopeptin,Cardionatrine,Cardiodilatin
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Atrial Natriuretic Peptide (ANP) ELISA Kit

The Human Atrial Natriuretic Peptide (ANP) ELISA Kit is specifically designed for the accurate measurement of ANP levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it an ideal tool for various research applications.ANP is a vital hormone involved in regulating blood pressure and fluid balance in the body. It is primarily produced in the atria of the heart and plays a key role in cardiovascular health.

By measuring ANP levels, researchers can gain valuable insights into conditions such as heart failure, hypertension, and other cardiovascular diseases, as well as potential therapeutic interventions.Overall, the Human ANP ELISA Kit offers a reliable and efficient method for studying the role of ANP in various physiological and pathological processes, making it a valuable tool for both basic research and clinical studies.

Product Name:Human Atrial Natriuretic Peptide (ANP) ELISA Kit
Product Code:HUDL00182
Size:96 Assays
Target:ANP
Synonyms:ANH, ANF, CDD, Atrial Natriuretic Factor, Atrial Natriuretic Hormone, Atriopeptin, Cardionatrine, Cardiodilatin
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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