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Human ATP citrate lyase / ACLY ELISA Kit

SKU:
HUFI01295
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P53396
Sensitivity:
0.938IU/ml
Range:
1.563-100IU/ml
ELISA Type:
Sandwich
Synonyms:
ACLY, ATP-citRate synthase, ATP-citRate, pro-S--lyase, ACL, CitRate cleavage enzyme, ATPCL, CLATP
Reactivity:
Human
Research Area:
Metabolism
€599
Frequently bought together:

Description

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Human ATP citrate lyase/ACLY ELISA Kit

The Human ATP Citrate Lyase (ACLY) ELISA Kit is a powerful tool for detecting and quantifying levels of ATP citrate lyase in human samples. ATP citrate lyase is a key enzyme involved in fatty acid synthesis and plays a crucial role in cellular metabolism.This ELISA kit offers high sensitivity and specificity, providing accurate and reliable results for research applications in areas such as cancer, obesity, and metabolic disorders.

By measuring ATP citrate lyase levels, researchers can gain insights into the role of this enzyme in disease progression and potentially identify new therapeutic targets.Overall, the Human ATP Citrate Lyase (ACLY) ELISA Kit is a valuable tool for studying the functions and regulation of ATP citrate lyase in various physiological and pathological processes.

Product Name:Human ATP citrate lyase / ACLY ELISA Kit
Product Code:HUFI01295
Size:96 Assays
Alias:ACLY, ATP-citRate synthase, ATP-citRate, pro-S--lyase, ACL, CitRate cleavage enzyme, ATPCL, CLATP
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human ACLY concentrations in serum plasma and other biological fluids.
Sensitivity:0.938IU/ml
Range:1.56-100IU/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human ACLY and the recovery rates were calculated by comparing the measured value to the expected amount of Human ACLY in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)95-10499
EDTA plasma(n=5)94-10496
UFH plasma(n=5)85-9592
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human ACLY and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)91-105%87-103%85-104%
EDTA plasma(n=5)84-98%86-101%88-99%
UFH plasma(n=5)86-93%80-100%86-97%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotP53396
UniProt Protein Function:ACLY: ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. Homotetramer. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:Protein type:EC 2.3.3.8; Transferase; Lyase; Carbohydrate Metabolism - citrate (TCA) cycle

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; cytosol; membrane; mitochondrion; nucleoplasm; plasma membrane

Molecular Function:ATP binding; ATP citrate synthase activity; cofactor binding; metal ion binding; protein binding

Biological Process: acetyl-CoA biosynthetic process; cellular lipid metabolic process; cholesterol biosynthetic process; citrate metabolic process; energy reserve metabolic process; fatty acid biosynthetic process; lipid biosynthetic process; oxaloacetate metabolic process; positive regulation of cellular metabolic process; triacylglycerol biosynthetic process

NCBI Summary:ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]
UniProt Code:P53396
NCBI GenInfo Identifier:116241237
NCBI Gene ID:47
NCBI Accession:P53396.3
UniProt Secondary Accession:P53396,Q13037, Q9BRL0, B4DIM0, B4E3P0,
UniProt Related Accession:P53396
Molecular Weight:91,099 Da
NCBI Full Name:ATP-citrate synthase
NCBI Synonym Full Names:ATP citrate lyase
NCBI Official Symbol:ACLY
NCBI Official Synonym Symbols:ACL; ATPCL; CLATP
NCBI Protein Information:ATP-citrate synthase
UniProt Protein Name:ATP-citrate synthase
UniProt Synonym Protein Names:ATP-citrate (pro-S-)-lyase; ACL; Citrate cleavage enzyme
Protein Family:ATP-citrate synthase
UniProt Gene Name:ACLY
UniProt Entry Name:ACLY_HUMAN

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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