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Human ATP Binding Cassette Transporter C8 (ABCC8) ELISA Kit

SKU:
HUDL00027
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q09428
Sensitivity:
0.128ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ABC-C8,PHHI,MRP8,ABC36,HHF1,HI,HRINS,SUR,SUR1,TNDM2,Sulfonylurea Receptor(Hyperinsulinemia)
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human ATP Binding Cassette Transporter C8 (ABCC8) ELISA Kit

The Human ATP-binding cassette transporter C8 (ABCC8) ELISA Kit is specifically designed for the accurate measurement of ABCC8 levels in human serum, plasma, and cell culture supernatants. This high-quality kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.ABCC8 is a key transporter protein involved in the regulation of cellular processes and the transport of various molecules across cell membranes.

Dysregulation of ABCC8 has been linked to diseases such as diabetes and cardiovascular disorders, highlighting its importance as a potential biomarker for studying these conditions and exploring novel therapeutic interventions.With its advanced technology and reliable performance, the Human ABCC8 ELISA Kit is an essential tool for researchers seeking to better understand the role of this critical transporter protein in health and disease.

Product Name:Human ATP Binding Cassette Transporter C8 (ABCC8) ELISA Kit
Product Code:HUDL00027
Size:96 Assays
Target:ABCC8
Synonyms:ABC-C8, PHHI, MRP8, ABC36, HHF1, HI, HRINS, SUR, SUR1, TNDM2, Sulfonylurea Receptor(Hyperinsulinemia)
Detection Method:Competition
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.Aspirate and wash 3 times
4.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.Aspirate and wash 5 times
6.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.Add 50µL Stop Solution. Read at 450 nm immediately.

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