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Human ATP Binding Cassette Transporter C4 (ABCC4) ELISA Kit

SKU:
HUDL00024
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O15439
Sensitivity:
0.118ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ABC-C4,MRP4,MOATB,Canalicular Multispecific Organic Anion Transporter B,Multidrug Resistance-Associated Protein 4,Multi-specific organic anion transporter B
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human ATP Binding Cassette Transporter C4 (ABCC4) ELISA Kit

The Human ATP-Binding Cassette Transporter C4 (ABCC4) ELISA Kit is specifically designed for the accurate detection of ABCC4 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.ABCC4, also known as the multidrug resistance protein 4, is a key transporter protein that plays a crucial role in mediating the transport of various substrates across cell membranes. Dysregulation of ABCC4 has been associated with drug resistance in cancer cells and is implicated in various diseases, making it a valuable biomarker for research and potential therapeutic development.

Overall, the Human ABCC4 ELISA Kit provides researchers with a reliable tool for studying the roles and mechanisms of this important transporter protein in health and disease. Its accurate and sensitive detection capabilities make it a valuable asset for advancing scientific understanding and potentially developing new treatment strategies.

Product Name:Human ATP Binding Cassette Transporter C4 (ABCC4) ELISA Kit
Product Code:HUDL00024
Size:96 Assays
Target:ABCC4
Synonyms:ABC-C4, MRP4, MOATB, Canalicular Multispecific Organic Anion Transporter B, Multidrug Resistance-Associated Protein 4, Multi-specific organic anion transporter B
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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